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KMID : 0350519940470041851
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1851 ~ p.1868
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Pre- and Post-mortem Metabolic and Morphologic Alteratons of the Dog Thalamus using Localized, Water-suppressed In Vivo H MR Spectroscopy and Electron Microscope
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Abstract
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Rationale and objectives of this study were to investigate the correlation of the postmortem metabolic and morphologic alterations in the left thalamus of the dog, and to contribute diagnostic aids in medicolegal science.
A homogeneous group of seventeen dogs was used for in vivo H magnetic resonance spectroscopy (H MRS) and electron microscope (EM) studies performed before slaughtering, and 0.5, 1, 1.5, 2, 2.5, 3, and 24 hours after slaughtering. H MRS was
performed by
1.5T MRI/MRS system using stimulated echo acquisition method (STEAM) pulse sequence, and tissue sections of the thalamus were examined under and electron microscope.
Lactate resonance signal indicating a criteria on life and death appeared in the whole postmortem period. The time-dependent postmortem metabolic changes of the thalamus demonstrated abruptly marked increase of lactate/creatine ratio from the 30
minutes
to the 3 hours period, however, it slightly decreased at the 24 hours. Inositol/creatine ratio presented significant increase up to postmortem 24 hours. Choline/creatine and N-acetylaspartate/creatine ratios were not significantly changed within
postmortem 24 hours.
EM study demonstrated that ultrastructural alterations of neurons in the nucleus ventralis anterior of the thalamus, marginal condensation of chromatin, cytoplasmic vacuoles, swollen mitochondria and Golgi complex, and dilated endoplasmic
reticulum
were
detected at postmortem 1 hour; more propressed cytoplasmic vacuolization at postmortem 3 hours; damaged mitochondria with fragmentation of the cristae at 12 hours; and clumping of nuclear chromatin, near-total destruction of the nuclear membrane,
cytoplasm filled with very large vacuoles and completely destructed cellular or ganelles in nerve cells, and fine honey-combed appearing sheets in myelinated nerve fibers at postmortem 24 hours.
The present results suggest that the combination of in vivo H MRS and EM may simultaneously
provide a better quality of metabolic and morphologic information of the brain tissue in doing research on thanatochronology.
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KEYWORD
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